Lynn Ferguson is a Lifestyle Therapist whose Specialty is in Bio-Energetic Medicine. She evaluated the Pearl

Powder Activated with Signal Protein using EAV equipment (Electro-acupuncture According to Voll). She has

training in Acupuncture and Herbs with knowledge of the value of pearl in formulations in Traditional Chinese

Medicine. She was sent a sample of the Pearlcium. This is based on three people's evaluations. This statement is from an email sent to TJ Johnson. She gave us permission to share.

Yes, the Pearl is THAT good!

Basically, so far it is a superior substance for regenerating the cellular structure of the human body, especially

the connective tissue, bone, tooth, nervous system, endocrine system, and circulatory system. It strengthens

the heart muscle, conduction system, and the vascular system, in addition to supporting the function of the

circulatory system. The Pearl rejuvenates the endocrine glands, in particular the pancreas, the adrenals, the

thyroid, the thymus, and improves pancreatic function for digestion while balancing both sluggish and

hyperactive adrenal function.

The Pearl improved and balanced the liver and kidneys, the bladder, lungs, and lymphatic system structurally

while supporting the function of these organs and strengthening the immune function in the lymph. The Pearl

balances the brain - both structure and function and supports the nervous system. The Pearl rejuvenates the skin and the eyes. It balances fluid metabolism and regulates xue (blood and body fluids like lymph,

cerebrospinal fluid, and synovial fluid). The activation of SOD (sodium oxide-dismutase) is one of the most

significant aspects of this product for those who are knowledgeable about such things.

That's an overview for now. This is just for three people and I will pass on more results as I continue to test others. I am of the opinion that

this just might be one of the most potent substances for balancing and regenerating the human body that I have

ever evaluated! Additional testing will provide more information, but at this rate everyone I test will either be

wanting to consume the product or market it or both.

Lynn Ferguson

I went to a Health Show last weekend and stopped at a booth that tests for bacteria buildup that causes

peridontal challenges. The attendant testing was absolutely amazed that she found no bacteria in my gum

tissue. The only thing that I could attribute this to is the Pearl Powder that I've been rubbing on my teeth and gums for the last 4 weeks. My skin is also much softer plus my fine lines are all diminishing. I am telling

everybody about this product!

Bernice G. CT

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Cut Sealed Itself In Minutes With No Sting

I severly cut my finger this week and nomally use my infrared tool to hold on any injury like this that works well, but it takes quite a few minutes to seal the cut. I grabbed the pearl powder to apply instead and IMMEDIATELY the bleeding stopped and also the burning sting was gone. WOW! Was I ever impressed. This product is incredible!

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The 6 year growth on my face has totally diminished in just a few days using the pearl nutrient powder topically (I only had a sample, not enough to take internally). I also had three sensitive teeth that hurt me on a constant basis, one molar really was throbbing so bad that I had to chew food on the other side of my mouth, all the sensive discomfort is totally gone. Also the discoloration on one of the teeth is fading.

My wife had a lesion under her left eye and it was red an inflamed, it has completely cleared up and the skin under the eye was really aged and wrinkled, now there are no wrinkles and the difference in her skin texture is profound. Her right eye is still wrinkled and leathery feeling, but the left eye has smooth skin where we applied the product. I also had a few scars on my right knee, most are gone and the others have shrunk by 80%.

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I tried the peral nutrients mixed with lotion for my knee discomfort last night and it did not hurt like

usual and I slept much better. I used 2 capsules and enough lotion for moisture.

Pearl Powder is a most welcomed suppplement . . . along with accupuncture --the synergy seems to be giving resolution to a jaw condition that I have had for a long time inspite of every approach /and base being explored and covered!!!!

In just one week taking the Pearl Nutrients internally and topically, my skin tags have diminished to almost half their original size. My energy levels have gone up and I've noticed more strength when a heavy steel garage door fell and I had to hold it for 30 minutes until help could arrive. I feel like Super Woman now!!

Pamela G. , AZ

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Whiter Teeth and Reversal Of Receding Gums

After only 2 weeks on the Pearl Powder Capsules, my teeth haven't been this white since I was 20. My 62 year old receding gums are filling in where the root of the tooth was becoming exposed and I have no discomfort in my right knee to kneel where I suffered a serious injury from a ski accident 21 years ago. I am also seeing an improved thickness in my skin on the tops of my hands and neck area. I can't believe what I am seeing in the  mirror!!

 Marjorie O. CA

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I have suffered with an acute breathing disorder for years and within a few weeks of taking the pearl nutrients, I experienced a significant improvement in my breathing. A serious wound on my arm that would not get better grew new healthy pink skin overnight. .My heart was beating irregulalry and now it is normal. This is amazing!

Living in Arizona, I have to be very careful to avoid the sun. I usually burn within a few minutes and develop sun poisoning. Now after taking the pearl powder capsules internally and applying it topically to my face, I can withstand several minutes in the sun and the redness disappears once in the shade. . . no adverse reactions now whatsoever. The Pearl Nutrients have a calming effect, as well as giving a feeling of well being. I love this product.

Marty P. AZ

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Foot and Skin & Eye Results

I have been suffering with hammer toes for years and finally had surgery to correct that disorder including some bunions removed that usually takes weeks for recovery. By taking 3 capsules orally and rubbing the pearl powder on my toes before bed, by the next morning I could wiggle my toes and bend my feet to get into shoes and I don't walk like a duck anymore. The most amazing results came from what this is doing with my 74 year old lines in my face. After applying the powder from the capsules topically with skin cream, I am constantly getting compliments now about my skin that is noticably smoother. . . . plus imporvement regarding a serious bruise that I recently acquired from a heavy planter that fell on my hand. The ugly black color disappeared overnight. It is now just a little red and this the fastest I have every reversed this type of bruise. I can't say enough good about this product.

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Last Tuesday, I had a hard fall after slipping off a curb. My right hip, shoulder and face took the brunt of the fall and I had bumps and bruises all over. I used the Pearl Nutrients as soon as I got home on my face plus all the areas that swelled. By the next day, I had no discomfort on those areas . . . only bruises that are also disappearing quickly.

I also suffered for years with a chronic itchy rash on my ankles, legs , fingers , and hips. The Pearl Powder stops the itch and clears up the redness. Usually I am so ashamed of my masses of red bumps, and sometimes scaly skin, and often weeping rash . I am careful to have my weeping rash covered because I am afraid of  redevelopting MRSA infection that I had in 1995. With the Pearl, my skin is cool to the touch now and not hot and inflammed, like it was. I make a Pearl Powder paste and apply it nightly to these areas of concern. Now I am not ashamed to wear a dress in public anymore like when I had to hide my "ugly" legs. I am very pleased with this product.

  Mary R, AZ

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I am only 19 years old, and I workout 5 days a week rather intensely.

As a result, I used to be very soar all over my body, every day. After taking

Pearlcium, my muscle soarness has drastically reduced, and I am expeieriencing

noticeable muscle growth from the Pearlcium as well. As with most teenagers, acne

has been an issue of mine, I have had above mild acne, and have been using the Pearlcium on my face as a

paste at night, and just brushing the powder on in the morning,

and the results I have seen have been terrific. Pearlcium hasn't dried my face at all, and as also helped with

acne scarring.

Kenneth B, FL.



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 Research on life prolonging effect 

It is not an easy task to scientifically confirm the ancient knowledge about the life prolonging effect of pearl powder in human beings.  It may take about 50 to 100 years. However, it is not so difficult to show this compelling evidence in animal. To be clear, Chinese scientists have shown that pearl powder can prolong the life span of animals in several research models.    

In one such study conducted in Suzhou Medical School, scientists found that by immersing some mulberry leaf, a favorite meal for silkworm’s, in a five percent pearl powder solution, that it can increase the adult phase of silkworm (moth) by an incredible 57.7 percent. When they fed mice their meals containing 1 percent pearl powder, they found that the mice’s life span on average was increased by 21.6 percent.

In another longevity study, scientists at Guangxi Chinese Medicine Research Institute studied the effect on fruit fly’s life span by feeding meals with some added pearl powder. They found that compared to the placebo group, the average life span of fruit flies fed with 0.5% pearl powder is increased from 55.2 days to 74.4 days for female fruit fly and from 45 days to 64.6 days for male fruit fly. Fruit flies’ longest life span is increased from 70 days to 100 days for female fruit fly and from 60 days to 102 days for male fruit fly.

If we were to translate the anti-aging effects, the profound life extending phenomena as observed in the silkworm, mice, and fruit flies from the pearl powder, it would indicate that our life span could increase from 80 years old to at least 96 years old on average. Perhaps, another compelling reason our ancestors found pearl powder so precious!

Traditional wisdom and current scientific research is demonstrating that pearl powder has a definitive anti-aging effect. More research and clinical trials are needed to confirm its full range of effects.  

 

2. Reduces wrinkles, aging, and sunspots and promotes radiant
and youthful-looking  

E. Lopez and other French scientists from the Biophysics Laboratory at the Museum D’Histoire Naturelle in Paris, France, have found that pearl nacre, which is the material made of pearl, can enhance regeneration of fibroblasts.⁸⁴ In their research, these scientists implanted pearl nacre in the dermis of rats to test the ingredient’s effect on skin fibroblasts. They found that pearl nacre enhanced the fibroblasts’ synthesis of the extracellular matrix. It increased the production of components that help cells stick together (which reinforces the barrier function of the skin) and their cellular communication with each other. And it also helped collagen - the main structural protein in the skin - regenerate itself.

This research scientifically confirms the traditional wisdom that pearl nacre can increase skin regeneration, thus help improve skin tone and promote youthful looking skin. It shows that pearl nacre does this by increasing collagen and other extracellular matrix production and by increasing cellular communication. However, it tells us more than this. Since fibroblasts are “builder cells” that abound in all connective tissue that surround our muscles, skin, and organs, by enhancing fibroblasts regeneration, pearl nacre in fact can help keep your body in shape and make your muscle and organs strong. This research may explain, at least partly, why pearl nacre can make your heart beat stronger and more regular and help improve eye health as being shown in other scientific research.  

3. Grow stronger bones by stimulating new bone growth and increasing bone density

In 1992, E. Lopez and other French scientists from the Biophysics Laboratory at the Museum D’Histoire Naturelle in Paris, France, did some interesting experiments to study the osteogenic properties of pearl nacre (again, this is the exact material that comprises both pearl and mother-of-pearl). ^56-59 They placed pearl nacre chips on a layer of human osteoblasts. They found that the osteoblasts that were near the pearl nacre chips proliferated, and then attached themselves to the chips. Even more amazing, the osteoblasts formed a complete sequence of bone in the presence of the pearl nacre. How do we know it was the pearl nacre that caused this? Because only the osteoblasts surrounding the pearl nacre chips induced this type of mineralization.

In 2003, the same group of French scientists decided to investigate pearl fillings. They placed pieces of mother-of-pearl in experimental cavities in the lumbar vertebrae of sheep. They found that the insertion of this pearl nacre induced the production of layers of newly formed bone adjacent to the implanted pearl nacre filling.⁵⁶ They also discovered that inserting the mother-of-pearl caused an increased mineralization of the sheep’s bone, which means that the bone surrounding the cavity actually became stronger.

Next, the scientists compared pearl-filled cavities with empty cavities and with cavities patched with the normal acrylic polymer filling used by dentists, which is called PMMA. They found there was no new bone formation in the empty cavities or in those filled with PMMA.⁵⁶ In fact, they discovered that PMMA actually causes necrosis – or cell death – of the surrounding bone cells. It also changes bone architecture and causes a significant reduction in bone formation and mineralization.

Dr. Y. Shen and colleagues studied the osteogenic activity of pearl in a culture of simulated body fluid and cells, with the intent to compare its effects to that of mother-of-pearl and hydroxyapatite, a form of calcium known to be osteogenic. They found that not only did pearl stimulate the growth of osteoblasts, but the proliferation occurred more quickly and smoothly than on either of the other substances.⁶⁰ In fact, an abundant extracellular matrix occupied the whole pearl surface after just five days. The researchers concluded that pearl is a superior osteo-inductive material with high osteogenic activity. In other words, pearl can build better bone than mother-of-pearl and hydroxyapatite.



4. Strengthen the heart, promote a regular heart rhythm, and aid recovery from heart disease

Research shows that pearl powder taken internally can improve heart function. Yunyi Zhang and colleagues at Shanghai Medical University studied pearl’s pharmacology and its effect on heart function.⁷⁷ They found that pearl powder can enhance the heart’s contraction without affecting the frequency of heart beat, which means more oxygen-rich blood is pumped per beat through the circulatory system without increasing numbers of beats. This means the heart is working smarter and more efficiently; not harder. The research also indicates that pearl powder can help the heart’s pacemaker keep its natural rhythm, helping rebuff and recover from the effects of arrhythmias or abnormal heart rhythm.



5. Promote healthy blood pressure

Kangmin Gong and his colleagues at Zhejiang Chinese Traditional Medicine Research Institute and Zhejiang University No. 1 Adjunct Hospital conducted a human clinical trial to study the effect of pearl powder on high blood pressure.⁷⁹ The 90 patients in the study took 500 milligram of pearl powder twice a day for 30 days. The average systolic pressure dropped from 161.0 mmHg to 139.2 mmHg – a decline of 15 percent. The average diastolic pressure dropped from 96.0 mmHg to 84.6 mmHg – a decline of 13 percent. 

In another similar human clinical trial with 75 patients, Kangmin Gong and his colleagues obtained similar successful results.⁸⁰ It has also been reported that patients notice fewer headaches, less dizziness and irritability, and improved sleep after taking pearl powder.

Dongmei Wang and her associates at Shaanxi Xian Jiaotong University Adjunct Hospital also studied pearl powder’s effects on blood pressure. They found that pearl powder can lower the blood pressure of mice, improve their sleep, and make them more calm.⁸¹

6. Promote healthy cholesterol levels

Blood lipid research indicates that pearl powder taken internally can reduce lipid peroxide and cholesterol levels of coronary disease patients.⁷⁸

Dr. Huang and his colleagues at Zhejiang Medical School conducted a double-blind, placebo-controlled human clinical trial to study the effect of pearl powder on lipid peroxides and cholesterol content of coronary disease patients. ⁷⁸ Twenty patients took pearl powder for a month. Seventeen patients took placebo. The researchers found that for the patients taking pearl powder, their harmful lipid peroxide and total cholesterol levels were significantly reduced, while for patients taking placebo, there was not much change on average. This can help stop the formation process of athroscelerosis in its track, before it can clog your arteries, so it never becomes a problem for your cardiovascular system.



7. Support healthy eye sight

H. Xu and colleagues at the Department of Chemistry of Huazhong University of Science and Technology provided scientific validation that pearl powder can help prevent and treat chickens with myopia.⁷⁶ 



8. Improve memory and immune system

Pearl powder might not only extend life span, it can also improve the quality of life. Studies done in China demonstrated that pearl powder can boost immune function in animals.^74,75  In one study, it found that taking pearl powder for 12 days can enhance mice’s antibody production and increase cellular protective functions.

Furthermore, it was found that after giving mice meals containing 1% pearl powder for 14 days, mice make much less mistake during study test.  This study indicates that pearl powder can improve learning ability and memory in mice. ⁷⁵ 



9. Calm nervous system, reduce stress and promote restful sleep

Jianxin Pan and his colleagues at Suzhou Medical School studied the effects of pearl powder on the rabbit’s nervous system.⁸⁹ They found that pearl powder could relieve pain and promote a calming effect on their nervous system. The researchers discovered also that after injecting the rabbits with a pearl powder solution, their brain production of 5-HT, 5-HIAA, and NA increased. These are positive “ elevated mood” neurotransmitters, and this research indicates that pearl powder can reduce the level of stress, and promote a more peaceful, positive internal biochemistry.  

 

10. Improve children's IQ, school performance, and overall health

Chinese scientists found that pearl powder can improve children’s IQ. In one study, they had more than 200 children with mental disabilities take 750-1500 mg of pearl powder every day. ⁹⁰ After three months, the IQ of 92% of the children has been improved and more than 80% of the children’s IQ were enhanced to normal range.

Dr. Jian Wang at Nanjin Second Hospital and Dehui Hou at China Medical University studied the effect of a pearl powder supplement on mice. They found that the mice that had taken pearl powder for 12 days did better in maze tests and had stronger immunity than the mice that did not.⁷⁴ In other words, pearl powder made the mice smarter and healthier.



11. Powerful antioxidant

Pearl Powder is a powerful antioxidant. It can both enhance the activity of Superoxide dismutase (SOD), one of the body's premier antioxidant enzymes, and also reduce peroxidation, one of the major aging processes of our body.

Shengshan Hu and his associates at Jiangxi Medicine Research Institute also studied the antioxidant effect of pearl nacre.⁵⁷ However, in addition to measuring lipid peroxidation (LPO) levels, they also monitored SOD activity. The researchers found that after taking pearl nacre solution for 30 days, the SOD activity of the mice increased and their LPO activity decreased.

Chinese researchers at Suzhou Medical School studied the effect of pearl powder on the content of peroxidized lipids in the mouse heart and brain.⁵⁷ These researchers found that after taking pearl powder for just 30 days, mice experienced a decrease in the amount of peroxidized lipids in their brains and hearts – indicating that pearl powder slows down and may even reverse some of the age-related damage. The results of this research were further confirmed in several other independent studies.^57-63

The less oxidation, the less damage to your vital organs, the more functioning and better metabolic balance you’ll have, with your chances for enhanced quality of life optimized.

Peroxidized lipids (LPO) are fats that have been attacked and damaged by free radicals. This is when even good fats lose their value, and get transformed into dangerous substances. Free radicals are the molecules that are highly unstable, and they can rapidly damage and degrade tissues that are not adequately protected. The more peroxidized lipids you have, the worse off you are. This is often used as a bio-marker for determining your accurate biologic health and true age beyond just counting your years. Unfortunately, your peroxidized lipid content usually increases with age; it is part of the normal aging process. That’s why there has been so much interest in antioxidants lately at well, as some antioxidants can prevent peroxidation, slow down pre-mature aging, and preserve more youthful functioning organs, skin, and even your good looks!

Superoxide Dismutase (SOD) is one of the body’s premier antioxidant enzymes. SOD is so important to your health and longevity, its manufacture is ensured by the body by being encoded into our DNA to make sure we make it for ourselves! SOD catalyzes the dismutation reaction, where a potent and potentially highly damaging superoxide free radical is rendered harmless and turned into simple oxygen and hydrogen peroxide. SOD eliminates superoxide radicals from the cell’s environment and prevents the formation of reactive oxygen species and their derivatives, as they are known cellular killers and cripplers. It plays an important role in maintaining vascular tone, lung function, and the metabolism, and in the prevention of such diseases as atherosclerosis, diabetes, and arthritis. The higher the SOD level is, the better it is for you. Many scientists consider SOD the key antioxidant player directly linked to longevity( closely coupled with Catalase and Glutathione), as various animal studies show higher SOD levels can support better health over a much longer life span.




12. Pearlcium is a superior source of calcium and essential trace minerals

In 1998, Xiaoping Li and his colleagues at Wuhan Mineralogy University in Wuhan, China, conducted human and animal studies comparing the bioavailability of pearl powder to that of other calcium supplements in a human clinical trial.⁵⁵ Their studies show that pearl powder is absorbed twice as efficiently as conventional calcium carbonate supplements (by far the most common form of calcium in dietary supplements).

Scientific tests reveal that calcium and essential trace minerals in pearl has a natural highly aligned micro-crystalline structure.

Latest scientific discovery reveals that the regulation of calcium involved in pearl formation is similar to humans’ at the DNA level. Our human body shares a deep kinship with pearl. Pearl is completely compatible with human bone. It can stimulate new bone growth, increases mineralization, which enhances bone density.



13. Pearl and osteoporosis 

The possibility of using pearl powder to treat animals with osteoporosis has been explored by Chinese scientists. Hongfu Wang and his associates from the Shanghai Medical School Senior Medicine Research Center studied pearl powder for the treatment of osteoporosis in mice.^53,54 Some mice were given a pearl powder formulation for 90 days; others were not. What the researchers discovered was that the mice that received the pearl powder formulation experienced significantly higher bone calcium content, bone mineral density, and total bone weight in comparison to the mice that did not. This is highly encouraging, but no guarantee, not yet anyway, as mice are used as the test subjects because they are excellent indicators for the expected effect in human metabolism. To date, pearl powder’s ability to treat osteoporosis in human’s is still clinically unknown, and we all await those human clinical studies for any confirmation of bone benefit.

14. Pearl and Immune System and Memory 

Pearl powder might not only extend life span, it can also improve the quality of life. Studies done in China also demonstrated that pearl powder can boost immune function in animals.^64,65  In one study, it found that taking pearl powder for 12 days can enhance mice’s antibody production and increase cellular protective functions.

Furthermore, it was found that after giving mice meals containing 1% pearl powder for 14 days, mice make much less mistake during study test.  This study indicates that pearl powder can improve learning ability and memory in mice. ⁶⁵ 




15. Pearl and eye sight 

For thousands of years, Chinese have used pearl to enhance their eyesight and treat eye diseases, such as myopia.  H. Xu and colleagues at the Department of Chemistry of Huazhong University of Science and Technology provided scientific validation that pearl powder made from pearl powder can help prevent and treat chickens with myopia.⁶⁶ 



 

References- 80 Medical Journals

1. Li S, Xie L, Zhang C, Zhang Y, Gu M, Zhang R.: “Cloning and expression of a pivotal calcium metabolism regulator: calmodulin involved in shell formation from pearl oyster (Pinctada fucata).” Comp Biochem Physiol B Biochem Mol Biol. 2004 Jul; 138(3):235-43.
2. Duplat D, Puisségur M, Bédouet L, Rousseau M, Boulzaguet H, Milet C, Sellos D, Van Wormhoudt A, Lopez E. “Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera.”, FEBS Lett. 2006 May 1;580(10):2435-41. Epub 2006 Apr 7.
3. Shen X, Belcher AM, Hansma PK, Stucky GD, Morse DE.: “Molecular cloning and characterization of lustrin A, a matrix protein from shell and pearl pearl of Haliotis rufescens.” J Biol Chem. 1997 Dec 19;272(51): 32472-81.
4. Zhang Y, Xie L, Meng Q, Jiang T, Pu R, Chen L, Zhang R. “A novel matrix protein participating in the pearl framework formation of pearl oyster, Pinctada fucata.” Comp Biochem Physiol B Biochem Mol Biol. 2003 Jul; 135(3): 565-73.
5. Tsukamoto D, Sarashina I, Endo K: “Structure and expression of an unusually acidic matrix protein of pearl oyster shells.” Biochem Biophys Res Commun. 2004 Aug 6;320(4):1175-80.
6. Michenfelder M, Fu G, Lawrence C, Weaver JC, Wustman BA, Taranto L, Evans JS, Morse DE.: “Characterization of two molluscan crystal-modulating biomineralization proteins and identification of putative mineral binding domains.” Biopolymers. 2003 Dec; 70(4):522-33.
7. Blank S, Arnoldi M, Khoshnavaz S, Treccani L, Kuntz M, Mann K, Grathwohl G, Fritz M., “The pearl protein perlucin nucleates growth of calcium carbonate crystals.” J Microsc. 2003 Dec; 212(Pt 3):280-91
8. Miyamoto H, Miyoshi F, Kohno J.: “The carbonic anhydrase domain protein pearlin is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata.” Zoolog Sci. 2005 Mar;22(3):311-5.
9. Yan Z, Fang Z, Ma Z, Deng J, Li S, Xie L, Zhang R, “Biomineralization: functions of calmodulin-like protein in the shell formation of pearl oyster.” Biochim Biophys Acta. 2007 Sep;1770(9):1338-44. Epub 2007 Jul
10. Takakura D, Norizuki M, Ishikawa F, Samata T., “Isolation and Characterization of the N-linked Oligosaccharides in Pearlin from Pinctada fucata.” Mar Biotechnol (NY). 2008 Feb 6
11. Wang N, Lee YH, Lee J., “Recombinant perlucin nucleates the growth of calcium carbonate crystals: Molecular cloning and characterization of perlucin from disk abalone, Haliotis discus discus.” Comp Biochem Physiol B Biochem Mol Biol. 2008 Feb;149(2):354-61. Epub 2007 Oct
12. Yan Z, Jing G, Gong N, Li C, Zhou Y, Xie L, Zhang R. “N40, a novel nonacidic matrix protein from pearl oyster pearl, facilitates nucleation of aragonite in vitro.” Biomacromolecules, 2007 Nov;8(11):3597-601. Epub 2007 Oct
13. Fan W, Li C, Li S, Feng Q, Xie L, Zhang R, “Cloning, characterization, and expression patterns of three sarco/endoplasmic reticulum Ca2+-ATPase isoforms from pearl oyster (Pinctada fucata)”, Acta Biochim Biophys Sin (Shanghai). 2007 Sep;39(9):722-30
14. Yano M, Nagai K, Morimoto K, Miyamoto H, “A novel pearl protein N19 in the pearl oyster Pinctada fucata.”, Biochem Biophys Res Commun. 2007 Oct 12;362(1):158-63. Epub 2007 Aug
15. Fan W, Li C, Wang X, Gong N, Xie L, Zhang R., “Cloning, characterization and expression analysis of calcium channel beta subunit from pearl oyster (Pinctada fucata).”, J Biosci Bioeng. 2007 Jul;104(1):47-54.
16. Metzler RA, Abrecht M, Olabisi RM, Ariosa D, Johnson CJ, Frazer BH, Coppersmith SN, Gilbert PU., “Architecture of columnar pearl, and implications for its formation mechanism.” Phys Rev Lett. 2007 Jun 29;98(26):268102. Epub 2007 Jun 29.
17. Xie LP, Wu YT, Dai YP, Li Q, Zhang RQ. “A novel glycosylphosphatidylinositol-anchored alkaline phosphatase dwells in the hepatic duct of the pearl oyster, Pinctada fucata.” Mar Biotechnol (NY). 2007 Sep-Oct;9(5):613-23. Epub 2007 Jul 13
18. Suzuki M, Sakuda S, Nagasawa H, “Identification of chitin in the prismatic layer of the shell and a chitin synthase gene from the Japanese pearl oyster, Pinctada fucata.” Biosci Biotechnol Biochem. 2007 Jul;71(7):1735-44. Epub 2007 Jul
19. Lin AY, Chen PY, Meyers MA, “The growth of pearl in the abalone shell.” Acta Biomater. 2008 Jan;4(1):131-8. Epub 2007 Jul 9
20. Ma Z, Huang J, Sun J, Wang G, Li C, Xie L, Zhang R, “A novel extrapallial fluid protein controls the morphology of pearl lamellae in the pearl oyster, Pinctada fucata.” J Biol Chem. 2007 Aug 10;282(32):23253-63. Epub 2007 Jun 8
21. Huang J, Zhang C, Ma Z, Xie L, Zhang R, “A novel extracellular EF-hand protein involved in the shell formation of pearl oyster.” Biochim Biophys Acta. 2007 Jul;1770(7):1037-44. Epub 2007 Mar 20
22. Liu HL, Liu SF, Ge YJ, Liu J, Wang XY, Xie LP, Zhang RQ, Wang Z, “Identification and characterization of a biomineralization related gene PFMG1 highly expressed in the mantle of Pinctada fucata.”, Biochemistry. 2007 Jan 23;46(3):844-51.
23. Naganuma T, Ogawa T, Hirabayashi J, Kasai K, Kamiya H, Muramoto K, “Isolation, characterization and molecular evolution of a novel pearl shell lectin from a marine bivalve, Pteria penguin.” Mol Divers. 2006 Nov;10(4):607-18. Epub 2006 Nov 17
24. Zhang C, Li S, Ma Z, Xie L, Zhang R., “A novel matrix protein p10 from the pearl of pearl oyster (Pinctada fucata) and its effects on both CaCO3 crystal formation and mineralogenic cells.”, Mar Biotechnol (NY). 2006 Nov-Dec;8(6):624-33. Epub 2006 Sep 18
25. Asakura T, Hamada M, Ha SW, Knight DP., “Conformational study of silklike peptides modified by the addition of the calcium-binding sequence from the shell pearlous matrix protein MSI60 using 13C CP/MAS NMR spectroscopy.” Biomacromolecules. 2006 Jun;7(6):1996-2002
26. Duplat D, Puisségur M, Bédouet L, Rousseau M, Boulzaguet H, Milet C, Sellos D, Van Wormhoudt A, Lopez E., “Identification of calconectin, a calcium-binding protein specifically
27. expressed by the mantle of Pinctada margaritifera.”, FEBS Lett. 2006 May 1;580(10):2435-41. Epub 2006 Apr 7
28. Li S, Xie L, Ma Z, Zhang R, “cDNA cloning and characterization of a novel calmodulin-like protein from pearl oyster Pinctada fucata.” FEBS J. 2005 Oct;272(19):4899-910
29. Wang XX, Xie L, Wang R., “Biological fabrication of pearlous coating on titanium dental implant”, Biomaterials. 2005 Nov;26(31):6229-32.
30.  Miyamoto H, Miyoshi F, Kohno J., “The carbonic anhydrase domain protein pearlin is expressed in the epithelial cells of the mantle and acts as a negative regulator in calcification in the mollusc Pinctada fucata.”, Zoolog Sci. 2005 Mar;22(3):311-5.
31. Li S, Xie L, Zhang C, Zhang Y, Gu M, Zhang R., “Cloning and expression of a pivotal calcium metabolism regulator: calmodulin involved in shell formation from pearl oyster (Pinctada fucata).”, Comp Biochem Physiol B Biochem Mol Biol. 2004 Jul;138(3):235-43
32. Tsukamoto D, Sarashina I, Endo K., “Structure and expression of an unusually acidic matrix protein of pearl oyster shells,” Biochem Biophys Res Commun. 2004 Aug 6;320(4):1175-80.
33. Suzuki M, Murayama E, Inoue H, Ozaki N, Tohse H, Kogure T, Nagasawa H, “Characterization of Prismalin-14, a novel matrix protein from the prismatic layer of the Japanese pearl oyster (Pinctada fucata)”, Biochem J. 2004 Aug 15;382(Pt 1):205-13
34. Matsushiro A, Miyashita T, Miyamoto H, Morimoto K, Tonomura B, Tanaka A, Sato K., “Presence of protein complex is prerequisite for aragonite crystallization in the pearlous layer.” Mar Biotechnol (NY). 2003 Jan-Feb;5(1):37-44
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38. Bédouet L, Marie A, Dubost L, Péduzzi J, Duplat D, Berland S, Puisségur M, Boulzaguet H, Rousseau M, Milet C, Lopez E., “Proteomics analysis of the pearl soluble and insoluble proteins from the oyster Pinctada margaritifera”, Mar Biotechnol (NY). 2007 Sep-Oct;9(5):638-49. Epub 2007 Jul 21.
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48. Lopez E, Vidal B, Berland S, Camprasse S, Camprasse G, Silve C. “Demonstration of the capacity of pearl to induce bone formation by human osteoblasts maintained in vitro.” Tissue Cell. 1992;24(5):667-79.
49. Lamghari M, Almeida MJ, Berland S, Huet H, Laurent A, Milet C, Lopez E. “Stimulation of bone marrow cells and bone formation by pearl: in vivo and in vitro studies.” Bone. 1999 Aug;25(2 Suppl):91S-94S
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53. Duplat D, Gallet M, Berland S, Marie A, Dubost L, Rousseau M, Kamel S, Milet C, Brazier M, Lopez E, Bédouet L. “The effect of molecules in mother-of-pearl on the decrease in bone resorption through the inhibition of osteoclast cathepsin K.” Biomaterials. 2007 Nov;28(32):4769-4778. Epub 2007 Aug 7 Hongfu Wang, Guangxin Zhou, Weifang Jin, Xingdi Li,
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55. Acta Academae Medicine Shanghai 25(1):43, 1998
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58. Shengshan Hu, Dayuan Wang, Jianpin Liu, Hong Yu, Hongwen Leng, Liwei Tan, Yuanzhen Xiong:”Research on anti-aging effects of pearl water solution.” Chinese Herbal Medicine 25(4): 203,  1994
59. Chen Y, Tan W, Qian Z., Gu Z., Pan J., Zhuang X.: “Experimental Study of the antioxidation effect of pearl” Senoir Medicine Journal 1988, 8(2): 109-120
60. Tong ZH, Gu WZ, Zhu G, Zhao YW. “The anti-aging effect of pearl oyster shell powder (POSP).” Journal of Traditional Chinese Medicine. 1988 Dec;8(4):247-50.
61. Yijun Chen, Tianxi Hu, Xucheng Chen: “Study of the antioxidation effect of pearl using chemical method.” Senoir Medicine Journal 8(6): 288, 1988
62. Zhi Xu, Derong Hu, Cai Cao, Huanying Wei, Shuhan Yao, Yuan Liu: “The effects of pearl on fruit fly’s life span and mice’s anti-radiation ability.” Chinese Medicine Journal 10(2): 29, 1998
63. Yijun Chen, WeishengTan, Zengping Qian, Zhenlun Gu, Jianxin Fan, Xinmei Zhuang: “Research on the anti-aging effects of pearl powder.” Senoir Medicine Journal 8(2): 199, 1988
64. Jian Wang, Dehui Hou: “The effect of pearl on free radical content and fruit fly’s life span.” Acta Academia Medicine Suzhou 17(2): 218, 1997
65. Jian Wang, Dehui Hou: “The effects of Pearl on animal’s immunity, learning and memory.” Acta Academiae Medicinal Suzhou 17(2): 216, 1997
66. Wang C, Ning X, Cheng J, Mo H., Song Z, “The effect of Pearl on Mice’s Immune Function”, Acta Academiae Medicine Guangxi 1994, 11(3): 280-282
67. Xu H, Huang K, Gao Q, Gao Z, Han X.: “A study on the prevention and treatment of myopia with pearl on chicks.” Pharmacol Res. Jul;44(1):1-6. 2001
68. Yunyi Zhang, Wenwu Gu, Xinrui Zhong: “The Pharmacology and Effects of Water Soluble Pearl on Heart Functions.” Chinese Medicine 16(9): 35, 1994
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71. Dongmei Wang, Yanping Song, Xifeng Hou, Junliang Gao: “Study of the pharmacology of Pearl Blood Pressure Lowering Medicine.” Shaanxi TCM Journal 23(4): 362, 2002
72. Daqing Zhou, Senlin Wu:”The antiinflammatary and antioxidant effects of pearl.” Journal of Zhejiang College of TCM 25(1): 41 (2001)
73. Chen Yijun, Zhaokang Yuan, “The study of the inhibition of tumor growth with Pearl porphyrins,” Modern Pharmacology Application Journal, 7 (6): 48, 1990
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75. Xinzhong Zhao, Zhemin Zhang, Jiqing Quan, Hongwei Qin: “fifty cases of using pearl to treat wrist cartilage.” Hebei Traditional Chinese Medicine Journal 13(5): , 1991
76. Linke Li, Yiyu Huang, Yin Chen: “The clinical study on using pearl to treat chronic mouth ulcer”, Journal of Beijing University of TCM 22(5): 66, 1999
77. Huang A., “The clinical study of pearl anti-burn paste”, Guangxi Medical Journal, 19 (1): 71, 1997
78. Wanfu Li, “The clinical study of using pearl formula to treat chronic skin ulcer”, Gansu Chinese Medicine Journal, 8(2): 10, 1997
79. Jianxin Pan, Zhenlun Gu, Zengnian Qian, Yajun Wang: “Study of the effects of pearl on central nervous system.” Chinese Traditional Medicine 21(11): 596, 1999
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